Zhao, Zhuangyu, Benjamin W. Roose, Serge D. Zemerov, Madison A. Stringer, and Ivan J. Dmochowski. “Detecting Protein–Protein Interactions by Xe-129 NMR.” Chemical Communications 56, no. 75 (2020): 11122–25.
https://doi.org/10.1039/D0CC02988B.
Detection of protein–protein interactions (PPIs) is limited by current bioanalytical methods. A protein complementation assay (PCA), split TEM-1 b-lactamase, interacts with xenon at the interface of the TEM-1 fragments. Reconstitution of TEM-1—promoted here by cFos/cJun leucine zipper interaction—gives rise to sensitive 129Xe NMR signal in bacterial cells.