Shcherbakov, Alexander A., João Medeiros-Silva, Nhi Tran, Martin D. Gelenter, and Mei Hong. “From Angstroms to Nanometers: Measuring Interatomic Distances by Solid-State NMR.” Chemical Reviews, October 25, 2021, acs.chemrev.1c00662.
https://doi.org/10.1021/acs.chemrev.1c00662.
Internuclear distances represent one of the main structural constraints in molecular structure determination using solid-state NMR spectroscopy, complementing chemical shifts and orientational restraints. Although a large number of magic-anglespinning (MAS) NMR techniques have been available for distance measurements, traditional 13C and 15N NMR experiments are inherently limited to distances of a few angstroms due to the low gyromagnetic ratios of these nuclei. Recent development of fast MAS triple-resonance 19F and 1H NMR probes has stimulated the design of MAS NMR experiments that measure distances in the 1−2 nm range with high sensitivity. This review describes the principles and applications of these multiplexed multidimensional correlation distance NMR experiments, with an emphasis on 19F- and 1H-based distance experiments. Representative applications of these long-distance NMR methods to biological macromolecules as well as small molecules are reviewed.