Ausmees, Kerti, Nele Reimets, and Indrek Reile. “Parahydrogen Hyperpolarization of Minimally Altered Urine Samples for Sensitivity Enhanced NMR Metabolomics.” Chemical Communications 58, no. 3 (January 4, 2022): 463–66.
https://doi.org/10.1039/D1CC05665D.
Parahydrogen hyperpolarization has been shown to enhance NMR sensitivity in urine analysis by several orders of magnitude if urine samples are prepared by solid phase extraction (SPE). We present a different approach, developed for minimal sample alteration before analysis. Removing SPE from the workflow allows to retain a wider range of metabolites and paves the way towards more universal hyperpolarized NMR metabolomics of low abundance metabolites.