Milani, Jonas, Felipe Saenz, Kelvin Lau, Florence Pojer, Sergey Nazarov, Bertrand Dominique Beckert, Alexander Myasnikov, Christophe Roussel, Holger Frauenrathe, and Jean-Philippe Ansermet. “A Method to Selectively Determine Concentrations of Macromolecular Complexes: Overhauser-DNP Spectroscopy.” Preprint. Chemistry, August 28, 2023.
https://doi.org/10.26434/chemrxiv-2023-c9kfd.
Dynamic nuclear polarization (DNP) is a process achieved by transferring the spin polarization from an unpaired electron to the half-spin nuclei in a substance of interest through microwave irradiation, effectively enhancing the nuclear magnetic resonance (NMR) signals of the substance. The polarization mechanism involved in Overhauser-DNP allows the process to be carried out in ambient conditions, thus facilitating its technological implementation. Here we show a method to quantify protein complexes in solution by exploiting the correlation among the concentration, the enhancement of the solvent NMR signals, the unpaired electrons’ motions, and the microwave irradiation frequency. As a proof of concept, we quantify the proteins avidin and C-reactive protein by means of adequate paramagnetic molecular probes (i.e., ligands), and we show that the method allows to estimate the dissociation constant for the C-reactive protein complex. Furthermore, we provide a full structural characterization of the avidin complex by X-ray crystallography (XRD) and cryogenic electron microscopy (cryo-EM).