Parker, Anna J., Arnab Dey, Mohammed Usman Qureshi, Jakob M. Steiner, John W. Blanchard, Jochen Scheuer, Nikolas Tomek, et al. “Solution‐state 2D NMR Spectroscopy of Mixtures Hyperpolarized Using Optically Polarized Crystals.” Angewandte Chemie International Edition, October 14, 2023, e202312302.
https://doi.org/10.1002/anie.202312302.
The HYPNOESYS method (Hyperpolarized NOE System), which relies on the dissolution of optically polarized crystals, has recently emerged as a promising approach to enhance the sensitivity of NMR spectroscopy in the solution state. However, HYPNOESYS is a single-shot method that is not generally compatible with multidimensional NMR. Here we show that 2D NMR spectra can be obtained from HYPNOESYS-polarized samples, using single-scan acquisition methods. The approach is illustrated with a mixture of terpene molecules and a benchtop NMR spectrometer, paving the way to a sensitive, information-rich and affordable analytical method.