Dear colleagues,
We are pleased to announce that the 15th Kiel Imaging Seminar (KIS) will take place on Monday, January 22nd, 18h Berlin time (GMT +2). We are much looking forward welcoming Prof. Björn Corzilius speaking on “Site-specific dynamic nuclear polarization in biomolecular MAS NMR”.
Who: Prof. Björn Corzilius, Institute of Chemistry, Dept. Physical Chemistry, Universität Rostock, Germany.
What: “Site-specific dynamic nuclear polarization in biomolecular MAS NMR”
When: Monday, January 22nd, 18h Berlin time (GMT +2)
Where: Zoom https://t.ly/cg47 (Meeting ID: 680 8649 6919, Passcode: 682584)
About the presenter:
Björn Corzilius is currently a professor for physical chemistry with focus on solid-state NMR spectroscopy at the Institute of Chemistry and the Department Life, Light & Matter at the University of Rostock. He studied Chemistry at the Technical University Darmstadt, Germany, where he received his Diploma in 2005 and his Ph.D. in 2008 under supervision of Prof. Klaus-Peter Dinse, studying the electronic properties of carbon nanomaterials by EPR spectroscopy. In 2009 he joined the group of Prof. Robert G. Griffin at the Francis Bitter Magnet Laboratory (MIT) where he investigated DNP mechanisms and developed new methods for biomolecular NMR applications. In 2013, he became an Emmy Noether research group leader at the Goethe University Frankfurt/Main, Germany, before being appointed to his current position in 2019.
About the presentation:
Solid-state NMR under magic-angle spinning (MAS) is an important tool in many scientific fields, including structural biology and materials science. However, NMR’s inherently low sensitivity often requires long acquisition times or even renders many experiments unfeasible. Dynamic nuclear polarization (DNP) can overcome this limitation by transferring much larger electron spin polarization to neighboring nuclei, thus enhancing their magnetization by up to several orders of magnitude. Typically, fast spin diffusion within the hyperpolarized ¹H bath is taken advantage of, in order to widely spread polarization over relatively large distances before transferring the ¹H polarization to heteronuclei for detection or further evolution. This results in a fast and uniform enhancement of the whole NMR spectrum.Interestingly, DNP also features the potential to site-specifically hyperpolarize nuclei and thus create spatial selectivity in the NMR spectrum. This is particularly useful in biomolecular MAS NMR where spectral crowding becomes a serious problem for large assemblies or complexes. In the presentation, one highly promising approach to create site-specific DNP enhancement is introduced: Specific cross-relaxation enhancement by active motions under DNP (SCREAM-DNP) can provide site-specific channels for spontaneous transfer of enhanced ¹H magnetization to heteronuclei in a hetNOE-type process. A detailed explanation will be given, and exemplary applications presented. Effects of uniform or sparse isotope labelling on nuclear spin relaxation, diffusion, and sitespecificity of DNP as well as limitations of the techniques will be discussed. In conclusion SCREAM-DNP has the power to yield spatially selective NMR spectra, for example, of sought-after biomolecular interfaces.
- Aladin et a. 2023. Specific Signal Enhancement on an RNA-Protein Interface by Dynamic Nuclear Polarization, Chem. Eur. J. 29, e202203443:1–12 (2023).
- Aladin et al. 2019. Complex formation of the tetracycline‐binding aptamer investigated by specific cross‐relaxation under DNP, Angew. Chem. Int. Ed. 58, 4863–4868 (2019).
We are looking forward to meeting you!
Your Section Biomedical Imaging
Dept. of Radiology and Neuroradiology,
University Hospital Schleswig-Holstein & MOIN CC, Kiel University
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KIS-email list: https://t.ly/fqyX
Zoom Link
https://t.ly/cg47
Meeting ID: 680 8649 6919
Passcode: 682584
About KIS: Invited speakers present their imaging topic in approximately 45 minutes, followed by some 15 minute discussion.
Recent Speakers List
Matthias Günther, Prof., (Fraunhofer MEVIS, D)
Jürgen Finsterbusch, Dr., (UKE Hamburg, D)
Robert Bok, Prof., (UCSF, USA)
Verena Hörr, Prof., (Herzzentrum Univ. Bonn, D)
Marian Hu, Dr., (Univ. Kiel, D)
Kevin M. Brindle, Prof., (Uni. of Cambridge, UK)
Vladimir Zhivonitko, Prof., (University Of Oulu, Fi)
Matthias van Osch, Prof., (Leiden Uni. Med. Cent., NL)
Helene Benveniste, Md, Prof., (Yale School of Med., USA)
Christoph Juchem, Prof., (Columbia Uni. New York, USA)
Ferdia Gallagher, Prof., (Uni. Of Cambridge, UK)
Seyma Alcicek, Phd., (Goethe Uni. Frankfurt, D)
Prof. Dr. Jan-Bernd Hövener
Head, Section Biomedical Imaging and MOIN CC
Head, Emmy Noether Group Molecular and Metabolic MRI - M3
Department of Radiology and Neuroradiology, UKSH
Kiel University
Section Biomedical Imaging, MOIN CC
Am Botanischen Garten 14
D-24118 Kiel
phone: +49 (0) 431 880-5832
fax: +49 (0) 431 880-5852
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