A Post-Doctoral position is available immediately to study functional amyloid formation by Streptococcus mutans, particularly as it relates to biofilm development. The focus of this project is to characterize the ultrastructure and amyloid properties of the adhesion P1 and other amyloidogenic surface proteins utilizing solution NMR, DNP-enhanced solid state NMR, cryoelectron microscopy, and diffraction techniques to study their structure in fibrillar and nonfibrillar forms. Applicants should have a strong background in biomolecular NMR spectroscopy, molecular microbiology, and biochemistry with an emphasis on protein structure/dynamics/function. Experience with standard cloning and recombinant DNA methodologies, protein chemistry, chromatography, and heterologous protein expression and purification is necessary. Familiarity with techniques to evaluate protein-protein interactions, protein crystallization and X-ray diffraction methodologies, mass spectrometry, and electron microscopy will be given preference. Also desirable would be familiarity with assays to assess amyloid fibrillization including use of fluorescent dyes and spectral shift assays, Congo-red induced birefringence, confocal microscopy and immunogold electron microscopy.
NMR facilities include fifteen spectrometers located at UF and the National High Magnetic Field Laboratory ranging from 500-900 MHz, including a 600 MHz ssNMR instrument with DNP capabilities. Extensive facilities for protein production and purification, cryoelectron microscopy, x-ray crystallography, and high performance computing are in close proximity to the Brady and Long labs as part of the UF centers for structural biology and high performance computing.
A Ph.D. and English language proficiency are required. Salary is commensurate with experience and range follows NIH guidelines. This is a grant-funded position and is annually renewable based on the availability of research funds. Please contact Joanna R. Long (jrlong@ufl.edu) and L. Jeannine Brady (jbrady@dental.ufl.edu) at The University of Florida, Departments of Biochemistry & Molecular Biology and Oral Biology, respectively, with any questions. (http://biochem.med.ufl.edu/research/faculty/primary-faculty/joanna-long/ and http://www.dental.ufl.edu/Offices/Oral_Biology/faculty/Brady_Linda/).
The University of Florida is an Equal Opportunity Institution dedicated to building a culturally diverse and inclusive faculty and staff. The selection process will be conducted in accord with the provisions of Florida’s “Government in the Sunshine” and Public Records Laws.
Joanna R. Long, PhD
Professor of Biochemistry & Molecular Biology
Director, Advanced Magnetic Resonance Imaging & Spectroscopy Facility
Assoc. Director, National High Magnetic Field Laboratory
McKnight Brain Institute, LG-187
Box 100245
University of Florida
Gainesville, FL 32605
(352)294-8399
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